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Bolton suffered a demoralising defeat to Shrewsbury Town in League One on Saturday, conceding twice in the final 11 minutes of the contest in Shropshire to suffer a 3-2 loss.
Ian Evatt‘s side led 2-1 until the 79th minute of the match, when Christian Saydee levelled, and Chey Dunkley then netted the winner for the home side in the 92nd minute.
The loss has left Bolton in fifth spot in the table, boasting 32 points from their 20 matches, with the Trotters looking to challenge for a return to the Championship this term.
Bolton finished top of Group B in the Northern Section, picking up seven points in the process, to advance to the next round, where they recorded a 3-2 victory over Barrow to progress to the quarter-finals.
The Trotters were knocked out of the FA Cup by Barnsley at the start of November, so this competition has taken on added importance, although the league remains the priority competition for the club
A number of the players have been away in Spain with the first team for their mid-season training camp, which included friendlies against Cadiz and Real Betis.
However, there is no reason, apart from injury, why the players should not be able to feature in this contest, so the Red Devils are likely to arrive with a strong squad on Tuesday night.
Man United Under-21s finished top of Group G of the Northern Section ahead of Barrow, with the team picking up seven points to progress to the round of 32.
Bolton boss Evatt will make changes to the side that started against Shrewsbury in the league on Saturday, with Joel Dixon likely to earn a start between the sticks.
Jon Dadi Bodvarsson should also feature in the final third of the field, with the striker on the scoresheet against Barrow in the last round.
However, Kyle Dempsey is set to miss out for the Trotters on Tuesday night through injury.
As for Man United Under-21s, head coach Dempsey will need to assess the fitness of the players who have been away with the first team in Spain.
Teden Mengi was taken off on a stretcher against Real Betis, though, with the centre-back now facing a period on the sidelines through injury.
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Jurado, Mainoo and Charlie Savage should among those to feature for the Red Devils, while Tom Huddlestone, who is a player-coach for the Under-21s, could start in central midfield.
Bolton Wanderers possible starting lineup:
Dixon; Toal, Ricardo, Iredale; Sadlier, Williams, Sheehan, John; Charles, Kachunga, Bodvarsson
Manchester United Under-21s possible starting lineup:
Bishop; Jurado, Bennett, Hardley, Murray; Mainoo, Huddlestone, Savage; Emeran, Shoretire, Gore
In the week that the Bulls lost their most famous ex-player, FA Cup legend Ronnie Radford – 50 years on from his iconic match-winning goal against Newcastle – it briefly looked like there might be some left-over magic in the air.
For seven minutes, the sixth-tier outfit led after Miles Storey’s goal against the team 81 places above them in the English football pyramid in League One.
But Pompey hit back, with Reeco Hackett quickly levelling before second-half goals from Colby Bishop and substitute Joe Pigott earned the twice FA Cup winners a safe passage to Monday’s second-round draw
Making their first trip to Edgar Street in 38 years, Pompey were given a good first-half workout by the fired-up hosts.
Although home keeper Dale Eve had to keep out Hackett’s left-foot curler bound for the top corner, Pompey had a first real scare when Bishop’s challenge on Hereford’s former million-pound marksman Tyrone Barnett in the visitors’ penalty box went unpunished.
And there was worse to follow for Pompey when Hereford took the lead on 26 minutes.
Sean Raggett played a loose pass inside to Hackett, who was immediately closed down and robbed by Storey – and the former Pompey player raced away down the inside-left channel before keeping his cool to nutmeg on-loan West Bromwich Albion keeper Josh Griffiths.
Fittingly, just two days on from the announcement of the sad passing of Hereford’s legendary number 11, their modern-day number 11 emulated his predecessor.
There had already been a minute’s silence, which cascaded into applause from the whole ground, followed by chants of ‘there’s only one Ronnie Radford’ when the clock showed 11 minutes.
But this goal was destined not to prove so famous.
Ronan Curtis headed down Connor Ogilvie’s far-post cross for Hackett to bring down and fire home coolly from six yards.
On 63 minutes, Portsmouth took the lead when, from Clark Robertson’s in-swinging right-foot corner from the left, Bishop timed his run to get there first and head his 10th goal of the season.
And Pigott wrapped up victory 10 minutes from time when, again from a corner by skipper Robertson, Bishop won the initial header before Pigott reacted sharpest to sidefoot home
Hereford FC boss Josh Gowling told BBC Sport:
“I’m really disappointed because we should have got something out of it, but goals change games – and the quality of the delivery they have changes games.
“In the first half we were excellent. We were in the ascendancy and they looked nervous.
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“We were on top and looked like we could go on and win, but we’ve made one mistake, it has cost us a goal and that’s the difference in levels.
“We’ve given them a leg up and a little lift and you could see their performance raise after that.”
Portsmouth boss Danny Cowley told BBC Sport:
“I was so sad to hear the news about Ronnie Radford. He was such an iconic figure and he had that iconic moment. For a lot of people my age, his famous goal was their first memories of the FA Cup. But what a brilliant night to celebrate his life